The singer suited up in a striped blazer and bow tie to perform his 2006 hit, "SexyBack." The barbershop quarter-style performance featured five men under the moniker "The Ragtime Gals" and also included vocals from Jimmy Fallon.
The late night host is known for remixing famous tunes! Last year, Mariah Carey performed "All I Want for Christmas Is You" alongside Fallon's house band, the Roots.
Check out the hilarious performance in the video below!
Watch The Ragtime Gals: "SexyBack" (w/ Jimmy Fallon & Justin Timberlake) Video
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